藏药“甲蒂(印度獐牙菜)”中龙胆苦苷和獐牙菜苷等10种成分的含量测定与质量评价(4)
YANG Yong1， LUO Wei-zao1， LIU Xiang1， WANG Chang-hua1， ZHAO Ji-feng1， QIN Song-yun1， ZHONG Guo-yue2*
(1. Chongqing Academy of Chinese Materia Medica， Chongqing 400065， China；(2. Jiangxi University of Traditional Chinese Medicine， Nanchang 330004， China)
[Abstract]Objective： To establish a method for determination of 10 ingredients such as gentiopicroside， sweroside， and mangiferin in India swertia， and settle the index components and their limits. Method： By Welch materials AQ-C18 column， determination was conducted by the gradient elution with methanol and 0.4% formic acid as mobile phase， with column temperature 30 ℃， flow rate at 1.0 mL·min-1， and 254 nm as the detection wavelength. Result： The linear relatives of 10 ingredients were good. The method showed the high precision and good reproducibility， and recovery rates were between 97% and 103%. The ingredients of market commodities varied greatly. Conclusion： This method is simple， sensitive， reproducible， and applicable to the determination of the main ingredients in India Swertia. Sweroside and mango glycosides were suggested as the index components for determination in Jia Di (Swertia chirayita)， and their content limits are not less than 0.1%，0.3%， respectively.
[Key words]Tibetan medicine； Jia Di； Swertia chirayita； HPLC， determination； limited content
doi：10.4268/cjcmm20122028
[责任编辑吕冬梅], 百拇医药(阳勇,罗维早,刘翔,王昌华,赵纪峰,秦松云, 钟国跃)
(1. Chongqing Academy of Chinese Materia Medica， Chongqing 400065， China；(2. Jiangxi University of Traditional Chinese Medicine， Nanchang 330004， China)
[Abstract]Objective： To establish a method for determination of 10 ingredients such as gentiopicroside， sweroside， and mangiferin in India swertia， and settle the index components and their limits. Method： By Welch materials AQ-C18 column， determination was conducted by the gradient elution with methanol and 0.4% formic acid as mobile phase， with column temperature 30 ℃， flow rate at 1.0 mL·min-1， and 254 nm as the detection wavelength. Result： The linear relatives of 10 ingredients were good. The method showed the high precision and good reproducibility， and recovery rates were between 97% and 103%. The ingredients of market commodities varied greatly. Conclusion： This method is simple， sensitive， reproducible， and applicable to the determination of the main ingredients in India Swertia. Sweroside and mango glycosides were suggested as the index components for determination in Jia Di (Swertia chirayita)， and their content limits are not less than 0.1%，0.3%， respectively.
[Key words]Tibetan medicine； Jia Di； Swertia chirayita； HPLC， determination； limited content
doi：10.4268/cjcmm20122028
[责任编辑吕冬梅], 百拇医药(阳勇,罗维早,刘翔,王昌华,赵纪峰,秦松云, 钟国跃)