枯草芽孢杆菌ge25菌株发酵及其抑菌活性成分的初步研究(4)
2. Institute of Medical Plant Development， Chinese Academy of Medicinal Sciences and
Peking Union College， Beijing 100193， China)
[Abstract] Panax ginseng is one of the most important traditional Chinese herbal medicine， soil borne diseases influenced the yield and quality severely. In our previous work， endophytic Bacillus subtilis ge25 strain was isolated from ginseng root， and which showed significant antagonistic activity against several most destructive ginseng phytopathogens. In the present work， crude protein and lipopeptid extracts were prepared from LB and Landy supernate by salting out， acid precipitation methods respectively. The antagonistic activity of crude extracts and stability to temperature and protease digestion were examined by ginseng phytopathogen Alternaria panax. Results showed that， the antagonistic activity of crude protein extracts from LB culture was complete and partially lost when treated by high temperature and proteinase K. However， crude lipopeptid from Landy culture showed significant stabile antagonistic activity to them. Acid-hydrolyzation and TLC-bioautography analysis showed， that the crude lipopeptide contained at least one cyclic lipopeptide. In consideration of the stability and perfect antagonistic activity of ge25， further researches will promote the biocontrol of ginseng diseases in the field.
[Key words] Panax ginseng； endophyte； B. subtilis； antagonistic substance； lipopeptid
doi：10.4268/cjcmm20141407
[责任编辑 吕冬梅], 百拇医药(胡陈云 李勇 刘敏 丁万隆 秦民坚)
Peking Union College， Beijing 100193， China)
[Abstract] Panax ginseng is one of the most important traditional Chinese herbal medicine， soil borne diseases influenced the yield and quality severely. In our previous work， endophytic Bacillus subtilis ge25 strain was isolated from ginseng root， and which showed significant antagonistic activity against several most destructive ginseng phytopathogens. In the present work， crude protein and lipopeptid extracts were prepared from LB and Landy supernate by salting out， acid precipitation methods respectively. The antagonistic activity of crude extracts and stability to temperature and protease digestion were examined by ginseng phytopathogen Alternaria panax. Results showed that， the antagonistic activity of crude protein extracts from LB culture was complete and partially lost when treated by high temperature and proteinase K. However， crude lipopeptid from Landy culture showed significant stabile antagonistic activity to them. Acid-hydrolyzation and TLC-bioautography analysis showed， that the crude lipopeptide contained at least one cyclic lipopeptide. In consideration of the stability and perfect antagonistic activity of ge25， further researches will promote the biocontrol of ginseng diseases in the field.
[Key words] Panax ginseng； endophyte； B. subtilis； antagonistic substance； lipopeptid
doi：10.4268/cjcmm20141407
[责任编辑 吕冬梅], 百拇医药(胡陈云 李勇 刘敏 丁万隆 秦民坚)