不同年龄拟巫山淫羊藿光合日变化研究(1)
[摘要] 淫羊藿是一种应用广泛的药用植物。因野生资源的过度利用而资源紧张，进行人工栽培是实现资源可持续利用的必由之路。拟巫山淫羊藿是首个实现人工栽培的物种，但种植生产技术尚未完全成熟。用CI340型光合测定系统原位测定了自然条件下生长的一、二、三年生拟巫山淫羊藿叶片光合作用的日变化，测量了光合速率(Pn)、气孔导度(C)、胞间CO2浓度(Ci)、光合有效辐射(PAR)、相对湿度(RH)、气温(Ta)、表观叶肉导度(ALMC)、蒸腾速率(E)、叶温(Tl)、水分利用效率(WUE)等光合生理因子和环境因子，并通过相关分析、通径分析和多元逐步回归分析探讨了净光合速率和生理生态因子的关系。结果表明：①拟巫山淫羊藿叶片净光合速率(Pn)、蒸腾速率(E)、表观叶肉导度(ALMC)、气孔导度(C)均呈双峰变化，其净光合速率具有典型的“午休”现象。②一年生植株的净光合效率、表观叶肉导度和气孔导度显著高于二、三年生；而胞间CO2浓度则基本上是三年生>二年生>一年生。③相关分析、通径分析和多元逐步回归分析表明，表观叶肉导度(ALMC)、胞间CO2浓度(Ci)、空气温度(Ta)是影响拟巫山淫羊藿光合速率主要的因子，影响大小的顺序为表观叶肉导度>胞间CO2浓度>空气温度。
[关键词] 拟巫山淫羊藿； 净光合速率； 通径分析； 相关分析； 多元逐步回归分析
Research on photosynthetic and ecophysiological characteristics of Epimedium pseudowushannense in different growing age
GU Xiaohong1，2， PAN Junqian2， YANG Xiangbo3， JIANG Zhihai3， LI Li3， GE Fei1， GUO Baolin2*
(1Jiangxi University of Traditional Chinese Medicine， Nanchang 330006， China；
2Institute of Medicinal Plant Development， Chinese Academy of Medical Science，Peking Union Medical College， Beijing 100193， China；
4Guizhou Tongjitang Pharmaceutical Co.， Ltd.， Guiyang 550002， China)
[Abstract] Epimedium is a widely used medicinal plants Due to excessive use of wild resources and resource constraints， artificial cultivation is the only way to achieve sustainable use of resources E pseudowushannense is intended to achieve the first cultivated species， but plant production technology is not yet fully matureThe diurnal variation of photosynthetic and ecophysiological characteristics of E pseudowushannense in different growing age was studied by the CI340 Portable Photosynthesis System under natural conditions The net photosynthetic rate (Pn)， stomatal conductance (C)， intercellular CO2 concentration (Ci)， photosynthetically active radiation (PAR)， relative humidity (RH)， air temperature (Ta)， apparent mesophyll conductance (ALMC)， transpiration rate (E)， leaf temperature (Tl)， water use efficiency (WUE) and other photosynthetic physiological factors and environmental factors were measured. The obtained data was analyzed by correlation analysis， path analysis and stepwise multipleregression analysis to explore the relationship between net photosynthetic rate and physiological and ecological factors The results show that： ①The daily variation of the Pn， E， ALMC， C of E. pseudowushannense in different growing age took on"two peak type"， the Pn was with an obvious midday depression phenomenon at noon②The Pn， ALMC and C of annuals’ were significantly higher than biennial or threeyear； but the Ci was basically a threeyear > biennial> annual③The correlation analysis， path analysis and stepwise regression analysis showed that ALMC， Ci， Ta might played important role in the Pn and ranked as ALMC>Ci>Ta (谷小红 潘俊倩 杨相波 姜志海 李砾 葛菲 郭宝林)
[关键词] 拟巫山淫羊藿； 净光合速率； 通径分析； 相关分析； 多元逐步回归分析
Research on photosynthetic and ecophysiological characteristics of Epimedium pseudowushannense in different growing age
GU Xiaohong1，2， PAN Junqian2， YANG Xiangbo3， JIANG Zhihai3， LI Li3， GE Fei1， GUO Baolin2*
(1Jiangxi University of Traditional Chinese Medicine， Nanchang 330006， China；
2Institute of Medicinal Plant Development， Chinese Academy of Medical Science，Peking Union Medical College， Beijing 100193， China；
4Guizhou Tongjitang Pharmaceutical Co.， Ltd.， Guiyang 550002， China)
[Abstract] Epimedium is a widely used medicinal plants Due to excessive use of wild resources and resource constraints， artificial cultivation is the only way to achieve sustainable use of resources E pseudowushannense is intended to achieve the first cultivated species， but plant production technology is not yet fully matureThe diurnal variation of photosynthetic and ecophysiological characteristics of E pseudowushannense in different growing age was studied by the CI340 Portable Photosynthesis System under natural conditions The net photosynthetic rate (Pn)， stomatal conductance (C)， intercellular CO2 concentration (Ci)， photosynthetically active radiation (PAR)， relative humidity (RH)， air temperature (Ta)， apparent mesophyll conductance (ALMC)， transpiration rate (E)， leaf temperature (Tl)， water use efficiency (WUE) and other photosynthetic physiological factors and environmental factors were measured. The obtained data was analyzed by correlation analysis， path analysis and stepwise multipleregression analysis to explore the relationship between net photosynthetic rate and physiological and ecological factors The results show that： ①The daily variation of the Pn， E， ALMC， C of E. pseudowushannense in different growing age took on"two peak type"， the Pn was with an obvious midday depression phenomenon at noon②The Pn， ALMC and C of annuals’ were significantly higher than biennial or threeyear； but the Ci was basically a threeyear > biennial> annual③The correlation analysis， path analysis and stepwise regression analysis showed that ALMC， Ci， Ta might played important role in the Pn and ranked as ALMC>Ci>Ta (谷小红 潘俊倩 杨相波 姜志海 李砾 葛菲 郭宝林)