原儿茶醛与羟基红花黄色素A单用与合用在高脂血症大鼠体内的药动学—药效学相关性研究(1)
[摘要] 探討原儿茶醛以及羟基红花黄色素A单用与合用在高脂血症大鼠体内的药动学及药效学相关性研究。该实验建立高脂血症大鼠模型，尾静脉注射液原儿茶醛(20 mg·kg-1)及羟基红花黄色素A(12 mg·kg-1)，采用HPLC-DAD测定不同时间内原儿茶醛和羟基红花黄色素A的血药浓度，并绘制药-时曲线；同时测定各时间点血浆中血小板活化因子(PAF)，血浆a颗粒膜蛋白(GMP-140)含量，绘制时间-效应曲线；采用DAS 3.2.6软件处理并进行相关性分析，比较分析单用及合用后原儿茶醛与羟基红花黄色素A在模型大鼠体内的药动学差异以及药效学指标的变化，评价原儿茶醛、羟基红花黄色素A在高脂血症大鼠体内的影响作用。结果原儿茶醛、羟基红花黄色素A在高脂血症大鼠体内单用及合用后，均符合三房室模型，模型组血浆中PAF及GMP-140明显升高，给予药物后在一定时间内降低PAF及GMP-140在体内的含量。原儿茶醛与羟基红花黄色素A抗药效指标的作用可能与其在体内的水平高低有关，并且其血药浓度与血浆中PAF及GMP-140的含量呈正相关。在高脂血症大鼠体内原儿茶醛和羟基红花黄色素A具有一定的互为影响作用，并且合用后药效学指标更好，同时也反映了原儿茶醛、羟基红花黄色素A合用在临床用药的合理性。
, 百拇医药
[关键词] 原儿茶醛；羟基红花黄色素A；高脂血症模型；药动学；药效学；联合用药
[Abstract] To study the pharmacokinetics-pharmacodynamics correlation of protocatechuic aldehyde and hydroxysafflor yellow A alone or their combination use in rats with hyperlipidemia. In this study， the hyperlipidemia model was established by intravenous injection of protocatechuic aldehyde (20 mg·kg-1) and hydroxysafflor yellow A (12 mg·kg-1). The HPLC-DAD method was applied to determine the plasma concentration of protocatechuic aldehyde and hydroxysafflor yellow A at different time points and draw the drug effect-time curve. Meanwhile， the platelet activating factors (PAF) and plasma a granule membrane protein (GMP-140) contents were determined at different time points to draw the time-effect curve. Then DAS 3.2.6 software was used to process the data， analyze their correlation， and compare the difference of pharmacokinetics and pharmacodynamics of protocatechuic aldehyde and hydroxysafflor yellow A in hyperlipidemia rats after alone or their combined application， so as to evaluate the effect of protocatechuic aldehyde and hydroxysafflor yellow A on hyperlipidemia rats. According to the result， the pharmacokinetics and pharmacodynamics process of protocatechuic aldehyde and hydroxysafflor yellow A in hyperlipidemia rats after alone or their combination were consistent to the three-compartment model. In model group， the plasma PAF and GMP-140 were significantly increased， and the PAF and GMP-140 in vivo contents were decreased in a certain time after treatment. The effects of protocatechuic aldehyde and hydroxysafflor yellow A against the pharmacodynamic action may be related with their level in vivo， and their plasma concentration was positively related to the PAF and GMP-140 contents. The pharmacodynamic indexes were better after the combined use of protocatechuic aldehyde and hydroxysafflor yellow A， with certain influence on each other in hyperlipidemia rats；at the same time， it also reflected the rationality of protocatechuic aldehyde and hydroxysafflor yellow A combined application., 百拇医药(范红晶 虞立 金伟锋 李敏 周静 李晓红 张宇燕)
, 百拇医药
[关键词] 原儿茶醛；羟基红花黄色素A；高脂血症模型；药动学；药效学；联合用药
[Abstract] To study the pharmacokinetics-pharmacodynamics correlation of protocatechuic aldehyde and hydroxysafflor yellow A alone or their combination use in rats with hyperlipidemia. In this study， the hyperlipidemia model was established by intravenous injection of protocatechuic aldehyde (20 mg·kg-1) and hydroxysafflor yellow A (12 mg·kg-1). The HPLC-DAD method was applied to determine the plasma concentration of protocatechuic aldehyde and hydroxysafflor yellow A at different time points and draw the drug effect-time curve. Meanwhile， the platelet activating factors (PAF) and plasma a granule membrane protein (GMP-140) contents were determined at different time points to draw the time-effect curve. Then DAS 3.2.6 software was used to process the data， analyze their correlation， and compare the difference of pharmacokinetics and pharmacodynamics of protocatechuic aldehyde and hydroxysafflor yellow A in hyperlipidemia rats after alone or their combined application， so as to evaluate the effect of protocatechuic aldehyde and hydroxysafflor yellow A on hyperlipidemia rats. According to the result， the pharmacokinetics and pharmacodynamics process of protocatechuic aldehyde and hydroxysafflor yellow A in hyperlipidemia rats after alone or their combination were consistent to the three-compartment model. In model group， the plasma PAF and GMP-140 were significantly increased， and the PAF and GMP-140 in vivo contents were decreased in a certain time after treatment. The effects of protocatechuic aldehyde and hydroxysafflor yellow A against the pharmacodynamic action may be related with their level in vivo， and their plasma concentration was positively related to the PAF and GMP-140 contents. The pharmacodynamic indexes were better after the combined use of protocatechuic aldehyde and hydroxysafflor yellow A， with certain influence on each other in hyperlipidemia rats；at the same time， it also reflected the rationality of protocatechuic aldehyde and hydroxysafflor yellow A combined application., 百拇医药(范红晶 虞立 金伟锋 李敏 周静 李晓红 张宇燕)