基于UPLCQTOF/MS技术的北青龙衣褐变过程中成分动态变化分析(1)
[摘要]该文分析不同褐变时期北青龙衣有效成分动态变化规律。采用UPLCQTOFMS/MS技术对13个褐变时期26批样品进行测定，差异性化合物通过Peakview 20/masterview10软件，依据精确质量数和同位素峰度比确定分子式，再通过与对照品及质谱数据库的二级谱图比对、裂解规律分析，并结合已有文献报道，确定结构式。结果不同褐变时期化学成分含量发生了明显的变化，鉴定或推断了北青龙衣中25个化合物结构，随着褐变时间的变化，13个萘醌类化合物中，具有胡桃醌相似母核的6个化合物及3个萘醌苷类化合物的含量显著下降，产生胡桃酮等4个萘醌衍生物；4个黄酮及2个酚酸类化合物含量显著下降；6个二芳基庚烷类化合物显著提高。该方法能够快速、准确鉴定北青龙衣的化学成分，并分析动态变化规律，为揭示褐变对中药材及果蔬成分的影响提供一种新的研究策略。
[关键词]液相色谱四极杆飞行时间质谱； 北青龙衣； 褐变； 动态变化
Dynamic variation of components in exocarp of Juglans mandshurica with
, http://www.100md.com
browning based on UPLCQTOF/MS
SUN Guodong， HUO Jinhai， XIE Rongjuan， WANG Weiming*
(Institute of Chinese Materia Medica， Heilongjiang Academy of Chinese Medicine Sciences， Harbin 150036， China)
[Abstract]To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods Twentysix batches of exocarp of J mandshurica samples from thirteen browning periods were assessed by UPLCQTOFMS/MS The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 20/masterview10 software Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred Of the 13 naphthoquinone compounds， the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly， and 4 naphthoquinone derivatives such as regiolone were produced； the contents of four flavones and two phenolic acids compounds were decreased significantly； and the contents of 6 diarylheptanoids compounds were increased significantly UPLCQTOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately， and analyze the rules of dynamic changes， to reveal the browning of Chinese medicinal materials and its effects on compositions of fruits and vegetables.
, 百拇医药
[Key words]LCQTOF/MS； exocarp of Juglans mandshurica； browning； dynamic variation
北青龍衣为胡桃科胡桃属植物核桃楸Juglans mandshurica Maxim未成熟果实的外果皮。民间多年来一直用于治疗恶性肿瘤，国内外学者对其抗肿瘤活性进行了大量的报道[12]。北青龙衣在鲜切过程中发生严重的褐变反应，由绿色逐渐变为黄绿色，干燥后变成棕褐色或黑褐色[34]。目前，关于褐变对北青龙衣化学成分及品质的影响研究尚未见报道。
植物经过一系列反应产生褐色聚合物的过程称褐变反应，简称褐变，分为酶促褐变和非酶促褐变。褐变是中药材、果蔬食品加工中普遍存在的一种变色现象，并严重影响其外观、营养价值及市场价格。近年来，国内外学者对百合、枸杞、葛根、山药、天麻、金银花、牡丹皮、苹果、大蒜、莲藕、荔枝、马铃薯等易发生褐变的药材、果蔬食品中褐变现象及其多酚氧化酶酶学特性进行了广泛而深入的研究[56]。, http://www.100md.com(孙国东 霍金海 谢荣娟 王伟明)
[关键词]液相色谱四极杆飞行时间质谱； 北青龙衣； 褐变； 动态变化
Dynamic variation of components in exocarp of Juglans mandshurica with
, http://www.100md.com
browning based on UPLCQTOF/MS
SUN Guodong， HUO Jinhai， XIE Rongjuan， WANG Weiming*
(Institute of Chinese Materia Medica， Heilongjiang Academy of Chinese Medicine Sciences， Harbin 150036， China)
[Abstract]To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods Twentysix batches of exocarp of J mandshurica samples from thirteen browning periods were assessed by UPLCQTOFMS/MS The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 20/masterview10 software Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred Of the 13 naphthoquinone compounds， the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly， and 4 naphthoquinone derivatives such as regiolone were produced； the contents of four flavones and two phenolic acids compounds were decreased significantly； and the contents of 6 diarylheptanoids compounds were increased significantly UPLCQTOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately， and analyze the rules of dynamic changes， to reveal the browning of Chinese medicinal materials and its effects on compositions of fruits and vegetables.
, 百拇医药
[Key words]LCQTOF/MS； exocarp of Juglans mandshurica； browning； dynamic variation
北青龍衣为胡桃科胡桃属植物核桃楸Juglans mandshurica Maxim未成熟果实的外果皮。民间多年来一直用于治疗恶性肿瘤，国内外学者对其抗肿瘤活性进行了大量的报道[12]。北青龙衣在鲜切过程中发生严重的褐变反应，由绿色逐渐变为黄绿色，干燥后变成棕褐色或黑褐色[34]。目前，关于褐变对北青龙衣化学成分及品质的影响研究尚未见报道。
植物经过一系列反应产生褐色聚合物的过程称褐变反应，简称褐变，分为酶促褐变和非酶促褐变。褐变是中药材、果蔬食品加工中普遍存在的一种变色现象，并严重影响其外观、营养价值及市场价格。近年来，国内外学者对百合、枸杞、葛根、山药、天麻、金银花、牡丹皮、苹果、大蒜、莲藕、荔枝、马铃薯等易发生褐变的药材、果蔬食品中褐变现象及其多酚氧化酶酶学特性进行了广泛而深入的研究[56]。, http://www.100md.com(孙国东 霍金海 谢荣娟 王伟明)